Black Reishi Liquid Culture Syringe

• Use potato dextrose agar (PDA) or malt extract agar (MEA) for initial culturing of the mycelium.
• Incubate Petri dishes at 24-27°C (75-80°F) in a dark environment until fully colonized.

• Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.
• Sterilize the grains and inoculate with the colonized agar from the Petri dishes.
• Incubate the grain spawn at 24-27°C (75-80°F) until fully colonized.

• Ganoderma sinense can be grown on hardwood sawdust or logs. Supplement the sawdust with bran or other nitrogen-rich materials to enhance growth.
• Ensure the substrate is well-moistened and properly sterilized to prevent contamination.

• Optimal temperature for mycelium growth: 24-27°C (75-80°F)
• Optimal temperature for fruiting: 18-24°C (64-75°F)

• Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.
• Mist the substrate regularly to maintain adequate moisture levels.

Ganoderma sinense requires light for proper development of fruiting bodies. Indirect sunlight or artificial light with a 12-hour light/dark cycle is recommended.

• Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.
• Ensure proper ventilation to maintain fresh air exchange and to avoid excess humidity that can lead to contamination.

• Colonization of the substrate typically takes 2-4 weeks, depending on environmental conditions.
• Fruiting can be expected within 4-6 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.
• Total growing time from inoculation to harvest is approximately 8-12 weeks.

The ideal pH range for cultivating Ganoderma sinense is between 5.5 and 6.5. Maintaining this slightly acidic environment helps optimize the conditions for the mycelium to thrive and produce fruiting bodies efficiently.