Pholiota adiposa, commonly known as the Chestnut Mushroom, is a captivating fungus notable for its rich chestnut-brown cap and clustered growth habit. It is prized both for its unique appearance and its culinary uses. This mushroom is often found growing on decaying hardwood, contributing to forest ecosystems by breaking down dead wood and returning nutrients to the soil.

Our Liquid Culture Syringe contains a pure, carefully isolated sample of mycelium nurtured and expanded in our sterile laboratory. This 10 mL/cc syringe is ideal for inoculating substrates with precision and ensuring rapid colonization. Each syringe is guaranteed to be contaminant-free, ensuring a high success rate for your cultivation projects. We offer a free replacement if needed.

Chestnut Mushroom Liquid Culture Syringe

Name: Chestnut Mushroom Liquid Culture Syringe
Brand: Mission Myco
SKU: MM-CM-LC-0001-10mL

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SKU: MM-CM-LC-0001-10mL

$20.00

$15.00

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Classification:

Kingdom: Fungi

Phylum: Basidiomycota

Class: Agaricomycetes

Order: Agaricales

Family: Strophariaceae

Genus: Pholiota

Species: P. adiposa

Other Names:

Chestnut Mushroom

Kuritake
Use potato dextrose agar (PDA) or malt extract agar (MEA) for initial culturing of the mycelium.

Incubate Petri dishes at 24-27°C (75-80°F) in a dark environment until fully colonized.
Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.

Sterilize the grains and inoculate with the colonized agar from the Petri dishes.

Incubate the grain spawn at 24-27°C (75-80°F) until fully colonized.
The optimal temperature for mycelium growth: 21-24°C (70-75°F)

The optimal temperature for fruiting: 15-18°C (59-64°F)
Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.

Mist the substrate regularly to maintain adequate moisture levels.
Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.

Ensure proper ventilation to maintain fresh air exchange and prevent contamination.
Colonization of the substrate typically takes 3-4 weeks, depending on environmental conditions.

Fruiting can be expected within 1-2 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.

Total growing time from inoculation to harvest is approximately 6-8 weeks.
For cultivating Pholiota adiposa, the ideal pH range of the substrate is typically between 5.5 and 6.5, with a slightly acidic environment being most conducive to growth. Maintaining this pH range helps optimize the conditions for the mycelium to thrive and produce fruiting bodies efficiently. Adjusting the pH of your substrate according to these guidelines can significantly impact the success of your Pholiota adiposa cultivation.