Portobello Liquid Culture Syringe

• Use malt extract agar (MEA) or potato dextrose agar (PDA) for initial culturing of the mycelium.
• Incubate Petri dishes at 24-27°C (75-80°F) in a dark environment until fully colonized.

• Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.
• Sterilize the grains and inoculate with the colonized agar from the Petri dishes.
• Incubate the grain spawn at 24-27°C (75-80°F) until fully colonized.

• A mixture of composted organic matter, such as horse manure, straw, and gypsum.
• Ensure the substrate is well-aged and properly composted to support vigorous mycelium growth.
• Maintain a substrate pH between 6.5 and 7.5 for optimal growth.

• Optimal temperature for mycelium growth: 24-27°C (75-80°F)
• Optimal temperature for fruiting: 18-22°C (64-72°F)

• Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.
• Mist the substrate regularly to maintain adequate moisture levels.

• Agaricus bisporus does not require light for mycelium growth.
• Low light conditions (indirect sunlight or artificial lighting) are beneficial during the fruiting stage to help the mushrooms orient themselves.

• Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.
• Ensure proper ventilation to maintain fresh air exchange.

• Colonization of the substrate typically takes 2-4 weeks, depending on environmental conditions.
• Fruiting can be expected within 1-2 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.
• Total growing time from inoculation to harvest is approximately 6-8 weeks.

Maintain a substrate pH between 6.5 and 7.5 for optimal growth. This slightly acidic to neutral environment is conducive to the healthy growth of Agaricus bisporus.