Bamboo Reishi Liquid Culture Syringe

• Use malt extract agar (MEA) or potato dextrose agar (PDA) for initial culturing of the mycelium.
• Incubate Petri dishes at 24-27°C (75-80°F) in a dark environment until fully colonized.

• Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.
• Sterilize the grains and inoculate with the colonized agar from the Petri dishes.
• Incubate the grain spawn at 24-27°C (75-80°F) until fully colonized.

• Ganoderma neo-japonicum can be grown on hardwood sawdust supplemented with bran, as well as on logs of hardwood trees like oak and maple.
• Ensure the substrate is well-sterilized to prevent contamination.

• Optimal temperature for mycelium growth: 24-27°C (75-80°F)
• Optimal temperature for fruiting: 18-24°C (64-75°F)

• Maintain high humidity levels of 85-95% during the mycelium growth and fruiting stages.
• Mist the substrate regularly to maintain adequate moisture levels.

• Ganoderma neo-japonicum requires indirect light during the fruiting stage to stimulate the development of fruiting bodies.
• Low to moderate light conditions are sufficient; avoid direct sunlight.

• Ensure good air circulation to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.
• Proper ventilation helps maintain appropriate humidity and temperature conditions.

• Colonization of the substrate typically takes 2-3 months, depending on environmental conditions.
• Fruiting can be expected within 1-2 months after the substrate is fully colonized and conditions are adjusted for fruiting.
• Total growing time from inoculation to harvest is approximately 4-5 months.

The ideal pH range for cultivating Ganoderma neo-japonicum is typically between 5.5 and 6.5. A slightly acidic environment is most conducive to growth.