Elm Oyster Tree Liquid Culture Syringe

• Use potato dextrose agar (PDA) or malt extract agar (MEA) for initial culturing of the mycelium.
• Incubate Petri dishes at 24-27°C (75-80°F) in a dark environment until fully colonized.

• Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.
• Sterilize the grains and inoculate with the colonized agar from the Petri dishes.
• Incubate the grain spawn at 24-27°C (75-80°F) until fully colonized.

• Elm Oyster Mushrooms thrive on hardwood sawdust, straw, or a mixture of both. Supplemented sawdust substrates with bran or other nitrogen-rich additives can enhance growth.
• Ensure the substrate is properly sterilized to avoid contamination and provide a nutrient-rich environment for the fungus.

• Optimal temperature for mycelium growth: 20-25°C (68-77°F)
• Optimal temperature for fruiting: 15-20°C (59-68°F)

• Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.
• Mist the substrate regularly to maintain adequate moisture levels.

• Elm Oyster Mushrooms require light for fruiting. Indirect sunlight or artificial lighting with a cycle of 12 hours of light and 12 hours of darkness is ideal.
• Light helps the mushrooms orient themselves and develop properly.

• Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.
• Ensure proper ventilation to maintain fresh air exchange and prevent contamination.

• Colonization of the substrate typically takes 2-4 weeks, depending on environmental conditions.
• Fruiting can be expected within 1-2 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.
• Total growing time from inoculation to harvest is approximately 6-8 weeks.

Maintain a substrate pH between 5.5 and 6.5 for optimal growth. This slightly acidic to neutral pH range is conducive to healthy mycelium development and fruiting body formation.