Flammulina velutipes, commonly known as Enokitake or Enoki mushroom, is a distinctive fungus known for its long, thin white stems and small, delicate caps. It grows naturally in clusters on the stumps of deciduous trees and is widely cultivated for its culinary uses, especially in East Asian cuisine. Enoki mushrooms are valued for their crunchy texture and mild flavor, and they are often used in soups, salads, and stir-fries. Additionally, they possess various medicinal properties, including antioxidant and immune-boosting effects.

Our Enokitake Liquid Culture Syringe contains a pure, carefully isolated sample of mycelium nurtured and expanded in our sterile laboratory. This 10 mL/cc syringe is ideal for inoculating substrates with precision and ensuring rapid colonization. Each syringe is guaranteed to be contaminant-free, ensuring a high success rate for your cultivation projects. We offer a free replacement if needed.

Enokitake Liquid Culture Syringe

Name: Enokitake Liquid Culture Syringe
Brand: Mission Myco
SKU: MM-E-LC-0001-10mL

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SKU: MM-E-LC-0001-10mL

$20.00

$15.00

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Classification:

Kingdom: Fungi

Phylum: Basidiomycota

Class: Agaricomycetes

Order: Agaricales

Family: Physalacriaceae

Genus: Flammulina

Species: F. velutipes

Other Names:

Enokitake

Enoki

Velvet Foot

Golden Needle Mushroom

Winter Mushroom
Use potato dextrose agar (PDA) or malt extract agar (MEA) for initial culturing of the mycelium.

Incubate Petri dishes at 20-25°C (68-77°F) in a dark environment until fully colonized.
Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.

Sterilize the grains and inoculate with the colonized agar from the Petri dishes.

Incubate the grain spawn at 20-25°C (68-77°F) until fully colonized.
Flammulina velutipes can be grown on a variety of substrates, including sawdust, straw, and agricultural waste. A mixture of sawdust and wheat bran or rice bran is commonly used.

Ensure the substrate is properly sterilized to avoid contamination.
Optimal temperature for mycelium growth: 20-25°C (68-77°F)

Optimal temperature for fruiting: 10-15°C (50-59°F)
Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.

Mist the substrate regularly to maintain adequate moisture levels.
Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.

Ensure proper ventilation to maintain fresh air exchange.
Colonization of the substrate typically takes 2-4 weeks, depending on environmental conditions.

Fruiting can be expected within 1-2 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.

Total growing time from inoculation to harvest is approximately 5-7 weeks.
For cultivating Flammulina velutipes, the ideal pH range of the substrate is typically between 5.0 and 7.0, with a slightly acidic to neutral environment being most conducive to growth. Maintaining this pH range helps optimize the conditions for the mycelium to thrive and produce fruiting bodies efficiently. Adjusting the pH of your substrate according to these guidelines can significantly impact the success of your Enoki cultivation.