Shaggy Mane Liquid Culture Syringe

• Use potato dextrose agar (PDA) or malt extract agar (MEA) for initial culturing of the mycelium.
• Incubate Petri dishes at 24-27°C (75-80°F) in a dark environment until fully colonized.

• Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.
• Sterilize the grains and inoculate with the colonized agar from the Petri dishes.
• Incubate the grain spawn at 24-27°C (75-80°F) until fully colonized.

• Coprinus comatus thrives on organic matter, including composted manure, straw, and sawdust. It can also grow on a mixture of straw and horse manure.
• Ensure the substrate is well-aged and properly composted to support vigorous mycelium growth.

• Optimal temperature for mycelium growth: 20-25°C (68-77°F)
• Optimal temperature for fruiting: 15-20°C (59-68°F)

• Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.
• Mist the substrate regularly to maintain adequate moisture levels.

• Shaggy Mane mushrooms do not require light for mycelium growth.
• Low light conditions (indirect sunlight or artificial lighting) are beneficial during the fruiting stage to help the mushrooms orient themselves.

• Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.
• Ensure proper ventilation to maintain fresh air exchange.

• Colonization of the substrate typically takes 2-3 weeks, depending on environmental conditions.
• Fruiting can be expected within 1-2 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.
• Total growing time from inoculation to harvest is approximately 4-6 weeks.

The ideal pH range for cultivating Coprinus comatus is typically between 6.5 and 7.5, with a slightly neutral environment being most conducive to growth. Maintaining this pH range helps optimize the conditions for the mycelium to thrive and produce fruiting bodies efficiently.