Lentinula edodes, commonly known as Shiitake, is one of the most popular and widely cultivated mushrooms in the world. Renowned for its rich, savory flavor and numerous health benefits, Shiitake mushrooms are a staple in many Asian cuisines and are increasingly appreciated globally for their nutritional and medicinal properties. They naturally grow on hardwood logs in forest environments but are now widely cultivated on sawdust-based substrates.

Our 1 lb. Shiitake Inoculated Grain Spawn is prepared using high-quality, sterilized grains and premium mycelium. This ready-to-use bag is perfect for inoculating bulk substrates or as a standalone medium for growing. Each bag is designed to ensure fast and robust mycelial growth, making it easy for novice and experienced cultivators to achieve great results.

Our 6 x 1 lb. Inoculated grain bags are convenient and cost-effective for larger cultivation projects. These bags are filled with sterilized grains and inoculated with high-quality mycelium, ensuring rapid and healthy colonization. This bulk pack is ideal for growers looking to produce at scale.

Our 12 x 1 lb. Inoculated grain bags provide an excellent value for large-scale cultivation. These bags are filled with sterilized grains and inoculated with high-quality mycelium, ensuring rapid and healthy colonization. This pack ensures you have a substantial supply to meet your growing needs.

Our 18 x 1 lb. Inoculated Grain Bags are designed for serious cultivators looking to maximize their production. These bags are filled with sterilized grains and inoculated with top-quality mycelium, ensuring vigorous and rapid colonization. This bulk pack offers the best value and ensures a continuous supply for larger grows.

Our Grain Spawn contains pure, carefully isolated samples of mycelium nurtured and expanded in our sterile laboratory. These grain spawn bags are ideal for inoculating a variety of substrates, ensuring rapid and even colonization. Each batch of grain spawn is guaranteed to be contaminant-free, ensuring a high success rate for your cultivation projects. We offer a free replacement if needed.

Shiitake Inoculated Grain Spawn

Name: Shiitake Inoculated Grain Spawn
Brand: Mission Myco

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From $15.30

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Classification:

Kingdom: Fungi

Phylum: Basidiomycota

Class: Agaricomycetes

Order: Agaricales

Family: Omphalotaceae

Genus: Lentinula

Species: L. edodes

Other Names:

Shiitake

Black Forest Mushroom

Golden Oak Mushroom

Oakwood Mushroom
Use potato dextrose agar (PDA) or malt extract agar (MEA) for initial culturing of the mycelium.

Incubate Petri dishes at 22-25°C (72-77°F) in a dark environment until fully colonized.
Grain spawn (oats, rye, millet, or sorghum) or sawdust spawn is recommended for inoculating the bulk substrate.

Sterilize the grains or sawdust and inoculate with the colonized agar from the Petri dishes.

Incubate the spawn at 22-25°C (72-77°F) until fully colonized.
Shiitake mushrooms can be grown on a variety of hardwood substrates including oak, beech, and maple. Hardwood sawdust supplemented with bran is commonly used for commercial cultivation.

Ensure the substrate is properly sterilized to avoid contamination and to provide a nutrient-rich environment for the fungus.
Optimal temperature for mycelium growth: 22-25°C (72-77°F)

Optimal temperature for fruiting: 10-20°C (50-68°F)
Maintain high humidity levels of 85-95% during the mycelium growth phase.

During fruiting, humidity should be kept around 75-85% to encourage the development of fruiting bodies and to prevent drying out.
Shiitake mushrooms require light to trigger and support fruiting. Low to moderate light conditions are sufficient. Indirect sunlight or fluorescent lighting can be used.

Light helps in cap development and ensures proper orientation of the mushrooms.
Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.

Ensure proper ventilation to maintain fresh air exchange, especially during the fruiting phase.
Colonization of the substrate typically takes 6-12 weeks, depending on environmental conditions.

Fruiting can be expected within 1-2 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.

Total growing time from inoculation to harvest is approximately 10-16 weeks.
For cultivating Shiitake mushrooms, the ideal pH range of the substrate is typically between 5.0 and 6.5, with a slightly acidic environment being most conducive to growth. Maintaining this pH range helps optimize the conditions for the mycelium to thrive and produce fruiting bodies efficiently. Adjusting the pH of your substrate according to these guidelines can significantly impact the success of your Shiitake cultivation.