Stropharia rugosoannulata, commonly known as the Wine Cap mushroom, is a robust and visually striking fungus appreciated for its large size, distinctive burgundy cap, and ease of cultivation. This mushroom is highly valued both for its culinary qualities and its role in garden sustainability. It naturally grows on wood chips and other lignin-rich substrates, making it an excellent choice for outdoor cultivation in garden beds and compost piles.

Our Wine Cap Liquid Culture Syringe contains a pure, carefully isolated sample of mycelium nurtured and expanded in our sterile laboratory. This 10 mL/cc syringe is ideal for inoculating substrates with precision and ensuring rapid colonization. Each syringe is guaranteed to be contaminant-free, ensuring a high success rate for your cultivation projects. We offer a free replacement if needed.

Wine Cap Liquid Culture Syringe

Name: Wine Cap Liquid Culture Syringe
Brand: Mission Myco
SKU: MM-WC-LC-0001-10mL

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SKU: MM-WC-LC-0001-10mL

$20.00

$15.00

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Classification:

Kingdom: Fungi

Phylum: Basidiomycota

Class: Agaricomycetes

Order: Agaricales

Family: Strophariaceae

Genus: Stropharia

Species: S. rugosoannulata

Other Names:

Wine Cap

Garden Giant

King Stropharia
Use malt extract agar (MEA) or potato dextrose agar (PDA) for initial culturing of the mycelium.

Incubate Petri dishes at 20-25°C (68-77°F) in a dark environment until fully colonized.
Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.

Sterilize the grains and inoculate with the colonized agar from the Petri dishes.

Incubate the grain spawn at 20-25°C (68-77°F) until fully colonized.
Stropharia rugosoannulata thrives on wood chips, straw, and other lignin-rich materials. Hardwood chips or a mixture of hardwood and straw are ideal substrates.

Ensure the substrate is pasteurized or sterilized to minimize contamination risks.
Optimal temperature for mycelium growth: 20-25°C (68-77°F)

Optimal temperature for fruiting: 15-20°C (59-68°F)
Maintain high humidity levels of 85-95% during the mycelium growth and fruiting stages.

Mist the substrate regularly to maintain adequate moisture levels, especially during dry periods.
Wine Cap mushrooms do not require light for mycelium growth.

Low to moderate light conditions are beneficial during the fruiting stage to help the mushrooms orient themselves and develop properly.
Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.

Ensure proper ventilation to maintain fresh air exchange and optimal growing conditions.
Colonization of the substrate typically takes 2-4 weeks, depending on environmental conditions.

Fruiting can be expected within 4-6 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.

Total growing time from inoculation to harvest is approximately 6-10 weeks.